Testing the Effectiveness of Natural Antibiotics

Objective

Evaluate the antibacterial properties of natural substances.

Materials

• Agar plates
• Bacterial cultures (e.g., E.coli)
• Natural antibiotics (e.g., garlic, honey, ginger)
• Sterile disks
• Incubator
• Notebook for observations

Instructions

1. Prepare agar plates and inoculate them with bacterial cultures.
2. Cut small disks from filter paper and soak them in the natural antibiotics.
3. Place the disks onto the agar plates.
4. Incubate the plates at 37°C for 24-48 hours.
5. Observe the zones of inhibition around each disk.
6. Measure the diameter of the inhibition zones and record in the notebook.
7. Compare the effectiveness of different natural antibiotics against the bacterial cultures.
8. Draw conclusions about which natural substance has the strongest antibacterial properties.